Unit 1

Instrumental Methods of Analysis

Instrumental Method of Analysis - Unit 1

UV Visible spectroscopy:

Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert's law, Derivation and deviations.

Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectorsPhoto tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.

Applications - Spectrophotometric titrations, Single component and multi component analysis

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Polymers:

Introduction, classification, properties, advantages and application of polymers in formulation of controlled release drug delivery

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Fluorimetry:

Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications

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